Camptothecin hl-60

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August undefined, 2024

WebNational Center for Biotechnology Information WebOct 1, 2003 · (A) HL-60 cells were treated with various doses of camptothecin, a DNA topoisomerase I inhibitor, for 20 hours. (B) H-7 cells were deprived of IL-3 for the indicated times. (Upper panels) Proteins were extracted and subjected to SDS-PAGE and Western blotting with anti-Bax antibody.

Caspase I-related protease inhibition retards the execution of …

WebFeb 1, 2008 · So, we conclude that rhPLD2 alone cannot induce apoptosis in HL-60 cells, but it can potentiate the apoptosis of HL-60 cells induced by camptothecin. Similarly, we show that both rhPLD2 and standard PLD were able to enhance camptothecin-induced apoptosis of HL-60 cells. Previousarticlein issue Nextarticlein issue Keywords rhPLD2 … WebAug 15, 2024 · The IC 50 values on the HL60 cells are comparable to SN38 and 9-N - (2′-hydroxyethyl)methyl substituted compound 2b. Table 1. IC 50 values of Irinotecan (1c), SN38 (1d) and SN38 derivatives 2a-j. nontoxic - lack of cell viability decrease/toxicity in the studied compound concentration range. irvine onnuri church

Figure 2. HL-60 cells treated with camptothecin for the …

WebCamptothecin is an alkaloid first isolated from the stem wood of the tree Camptotheca acuminata (Nyssaceae), and has potent antitumor activity. 31 Camptothecin acts by … Web周珏，卢林明，张根葆(皖南医学院 1.病理解剖学教研室;2.病理生理学教研室;3.蛇毒研究所，安徽芜湖 241002)蛇毒具有广泛的生 WebApr 27, 2010 · Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT). We used bivariate bromodeoxyuridine/propidium … irvine onnuri church website

Apoptosis induced by the homocamptothecin anticancer …

Polysaccharopeptides derived from - Chinese Medicine

WebThe meaning of CAMPTOTHECIN is an alkaloid C20H16N2O4 from the wood of a Chinese tree (Camptotheca acuminata of the family Nyssaceae) that has shown some … WebJun 1, 1992 · Camptothecin or teniposide induced apoptosis selectively in S phase of HL-60 cells; apoptotic cells from camptothecin-treated cultures, however, despite the marked nucleolysis, still expressed p120. irvine online internetWebThis potent antioxidant is a cytoprotectant in several experimental models. However, its neuroprotective activities are scarcely available, especially in the hippocampal region associated with... portchester news uk

"WebJun 1, 2000 · Camptothecin (50 mM), a cytotoxic quinoline alkaloid which inhibits the DNA enzyme topoisomerase I, was used as a positive control (King et al., 2000). ... Internalization of Bacillus intermedius... " - Camptothecin hl-60

Camptothecin hl-60

Polysaccharopeptides derived from - Chinese Medicine

WebJan 15, 2024 · In summary, 12 novel biotinylated camptothecin derivatives were designed, synthesized, and evaluated for anticancer activities against a panel of five human cancer … WebSep 1, 2001 · To shed light on the mechanism of action of hCPT at the cellular level, we compared the effects of BN80915 and the classic camptothecin SN-38, the active metabolite of irinotecan, on HL-60 human promyelocytic cancer cells.

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WebThere have been some conflicting results concerning whether annexin V binds to camptothecin (CAM)-treated HL-60 cells, a commonly used model for apoptosis. We investigated the effects of culturing HL-60 cells for up to 8 h with a range of CAM concentrations. METHODS We used flow cytometry to measure cellular light scatter, … WebCamptothecin is a naturally occurring alkaloid derived from the plant Camptotheca acuminata that was identified in an anticancer drug discovery screen in the 1960s. 68 …

WebJun 29, 2015 · ；D. 大黄素无法捕获Topo I-DNA 的可切割复合物，Topo 的抑制剂CPT作为阳性对照；大黄素能够诱导稳定的TopoII α-DNA 可切割复合物，Topo II 的抑制剂VP16 作为阳性对照。G. 用大黄素处理HL-60 和HL-60/MX2 细胞后，检测γ -H2AX 的表达。H. 大黄素和VP16在Topo II –缺陷型细胞HL ... WebOct 21, 2024 · New 2,3-disubstituted thieno[2,3-d]pyrimidin-4(3H)-ones were synthesized via a one-pot reaction from 2H-thieno[2,3-d] [1,3]oxazine-2,4(1H)-diones, aromatic aldehydes, and benzylamine or 4-hydroxylbezylamine. The obtained compounds were tested in vitro for cancer cell growth inhibition. Compound 19 can inhibit all four types of tested cancer …

WebJun 13, 2024 · Camptothecin is an anticancer molecule which acts by targeting the human deoxyribonucleic acid topoisomerase I (Topo-I). It inhibits the enzyme by blocking the re-joining step of the cleavage reaction of Topo-I, which causes accumulation of a covalent reaction intermediate. WebWe investigated expression of cyclin A in HL-60 cells after induction of apoptosis with doxorubicin and etoposide. Following apoptotic trigger, both cells arrested in G2/M phase of the cell cycle...

WebDNA single- strand breaks were detected using alkaline sucrose gradient centrifugation when HL-60 cells were incubated with 10 μMCPT or 10 μMSN-38 for 30 min. These …

WebNov 1, 2002 · The role of mitochondria in HL-60 apoptosis was explored using an inhibitor of oxidative phosphorylation, antimycin A (AMA). Methods: Changes in cell light scatter, … portchester middle school websiteWebAug 26, 2002 · Camptothecin (CAM; Sigma, St. Louis, MO) initiates apoptosis in the human promyelocytic leukemia cell line, HL-60, via its binding to topoisomerase I–DNA … portchester opticiansWebFeb 1, 2008 · In the present study, we investigated the role of recombinant human phospholipase D2 (rhPLD2) on proliferation and apoptosis in human leukemia HL‐60 cells which induced by camptothecin. Our research demonstrated that various concentrations of rhPLD2 inhibit the growth of HL‐60 cells in a dose‐dependent manner, and rhPLD2 plus … portchester hotelsWebFounded. 1932. Founder. Harold L. Green. Defunct. 1990s. Fate. Closed as part of parent company ( McCrory Stores) bankruptcy in 2001. H. L. Green was a five and dime store … portchester oak coretecWebFeb 1, 2008 · Camptothecin HL-60 cells Apoptosis Cell cycle 1. Introduction Phospholipase D (PLD) is an enzyme that hydrolyzes phospholipids. In mammalian cells, there are two PLD isoforms, PLD1 and PLD2 that share significant sequence similarity, but have distinct subcellular locations and activity. portchester oak coretec flooringWebThe Aerospace Maintenance Competition is an annual celebration of aviation maintenance technicians and their skills. Held during Aviation Week at the MRO Ame... irvine orthodontics and children\u0027s dentistryWebHL-60 cells exposed to okadaic acid and camptothecin underwent morphological and biochemical changes typical of apoptosis, including internucleosomal DNA fragmentation and PAI-2 cleavage. portchester parish hall hire