Tritiated thymidine incorporation
WebInhibition of tritiated thymidine ([3H]dThd) incorporation into replicating DNA of concanavalin A (Con A)-stimulated C57BL/6J splenic lymphocytes has been further evaluated as an end point for rapid in vitro discrimination between carcinogenic and noncarcinogenic chemicals. Results of this evaluatio … WebThymidine incorporation is highly predictive of colony formation and can be used for high-throughput screening. The primary goal of anticancer chemotherapy is to kill cancer cells. …
Tritiated thymidine incorporation
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Webthe body. Tritiated water and readily metabolizable forms are eliminated with a 10-day biological half-life. Elimination rates can be increased by increasing water intake. Some special cases exist, notably tritiated thymidine, which can result in higher doses to the cell nucleus due to its specific incorporation into DNA. Since DNA has low ... WebAfter 48–72 h of culture, tritiated thymidine is added to culture wells and the incorporated radioactivity is measured 24 h later. Bromodeoxyuridine (BrDU) can be used as a nonradioactive alternative to tritiated thymidine and BrDU incorporation is …
WebJul 1, 2014 · Given the interests of the developers of [3 H] thymidine, it is understandable that there was immediate interest in using it to study the effects of ionizing radiation (16 – 18). Radiation-induced suppression of [3 H] thymidine incorporation demonstrated the vulnerability of the cell cycle and DNA synthesis to relatively low doses. These ... WebJun 1, 1991 · The U.S. Department of Energy's Office of Scientific and Technical Information
WebWhen human lymphocytes were cultured with [ 3 H]thymidine, which acts as a source of low-level chronic radiation, and then exposed to 150 rad of x-rays at 5, 7, 9, or 11 hours before fixation, the yield of chromatid aberrations was less than the sum of the yields of aberrations induced by [ 3 H]thymidine and x-rays separately. WebTritiated thymidine incorporation and the growth of heterotrophic bacteria in warm core rings (Journal Article) OSTI.GOV skip to main content Sign In Create Account Show searchShow menu U.S. Department of EnergyOffice of Scientific and Technical Information Search terms:Advanced search options
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Web248 Argyle St S, Renfrew, ON K7V 1T7 Get directions. Labour Law, Commercial Law, Commercial Law, Construction Law, Trust Law, Taxation Law, Civil Law, Collaborative … coffee shop old marketWebFeb 28, 2014 · As a co-author of one of two papers published in Radiation Research [1,2] that deal with the survival kinetics of Chinese hamster cells exposed to tritiated thymidine, as a member of the research group here involved and as co-investigator on the NIH grant that supported the research, one of us (Helene Z. Hill) became concerned that certain … coffee shop old orchard beachWebWhen the incorporation of tritiated thymidine into acid insoluble material was measured, ribavirin appeared to be a potent inhibitor of DNA synthesis in KB cells and human lymphocytes. Inhibition was nearly 100-fold less, however, when DNA synthesis was measured by incorporation of phosphorus-32-labeled phosphate or by DNA fluorescence. coffee shop old town conyersWebTritiated thymidine does not seclusive enter the bacterial DNA and several studies have demonstrated the labelling of macromolecular compounds other than DNA (Hollobaugh … cameron heacock 2022WebFeb 21, 2024 · To do so, tritiated thymidine, an organic form of tritium, was chosen and zebrafish embryos [3.5 h post fertilization (hpf)] were exposed to a range of activity concentrations (2.21 × 103 to 5.95 × 105 Bq/mL). ... indicating that random incorporation of the thymidine analog Brd U into the genes of dividing cells makes the fate of postmitotic ... coffee shop old town fort collinsWebThe T cell response to immunogenic peptides presented by dendritic cells (DC) was assessed using 3 H thymidine incorporation as a read-out. After loading the DCs with the peptides, T cells were added to the culture. … coffee shop on 91st between 2nd and 3rdWeb[3 H]-thymidine incorporation. This well-validated semiquantitative assay uses tritiated [3 H]-thymidine incorporation into nascent DNA synthesized during the S phase of cell cycle, to … coffee shop old town scottsdale